Abstract
A total of 32 non-O157 diarrheagenic Escherichia coli isolated (Miri hospital, Sarawak, Malaysia, 2001) from the patients with diarrhea were examined for the detection of verotoxin (VT). Two sets of primers (VT1 and VT2) were used for PCR method, which produced a fragment of 348 bp of VT1 gene and 584 bp of VT2 gene, respectively. It was shown that 13 E. coli isolates (40%) carried VT1 gene whereas none was found to carry VT2 gene using PCR protocols. Plasmids were detected in all the VT1-positive isolates. From the Southern hybridization study, VT1 probe showed homology with the chromosome of all 13 VT1positive isolates indicating the VT1 gene to be located on chromosome. Bacteriophage induction as carried out using mitomycin C showed that none of the VT1-positive isolates harbor any lysogenic bacteriophage. However, nucleotide sequencing made from the VT1 gene fragment isolated from an E. coli isolates using PCR protocol showed 97% homology with the known VT1 gene which proves to be the similar gene carried by E. coli O157 and other ancestors.
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