Abstract
It has been suggested thatduring myocardial ischemia short-lived free radicals such as superoxide (O 2 - ·), hydroxyl (·OH), or lipid peroxide (RO.) are produced. The evidence, however, of the participation of such radicals in cardiovascular injury has been, for the most part, based upon activation of lipid peroxidation and on myocardial protection provided by scavengers.1,2 Direct detection of such radicals, as might be possible by electron spin resonance spectroscopy (ESR) has not provided conclusive identification of radicals produced during these injuries. However, spin trapping and ESR offer an indirect technique that provides the evidence necessary to confirm the involvement of these labile intermediates. Spin trapping has been applied very successfully to studies of free radical mechanisms in a variety of chemical and photochemical reactions; however, it has only recently been applied to biological systems. The majority of the spin traps being used are molecules containing a nitroso or nitrone group which, upon addition of a labile free radical, form a stable nitroxide that is easily detected by ESR.
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