Abstract
PPR also known as goat plague is an acute highly contagious disease of sheep and goats characterized by fever, catarrhal inflammation of ocular and nasal mucous membrane and diarrhea. The present study was conducted for molecular detection and isolation of Peste des petits ruminants (PPR) virus in sheep and goats in Punjab region. A total of 150 samples; ocular (25), nasal (25) and rectal swabs (25) separately from sheep and goats were collected. These samples were subjected to Reverse Transcriptase-PCR for molecular detection of PPR virus. Out of 75 samples from 25 goats, three samples (4%) were found positive for F gene by RT-PCR and all the samples from sheep were negative for PPR virus. PPR virus was isolated from one sample using vero cell line.
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