Abstract
A gas chromatographic method has been developed for the detection and identification of some volatile organic compounds in whole blood, plasma or serum. After incubation of the sample (200 μl) together with the internal standard solution in a sealed vial, a portion of the headspace in analysed using a 2-m glass column packed with 0.3% (w/w) Carbowax 20M on Carbopack C, 80–100 mesh. The column oven, after a 2-min isothermal period, is programmed from 35 to 175°C at 5°/min and held for 8 min. the effluent is monitored by both flame-ionisation and electron-capture detection, and peak assignment is by means of retention time and relative detector response. The method has proved applicable to the detection of bromchlorodifluoromethane, n-butane, carbon tetrachloride, chlorobutanol, cryofluorane (Halon 114), dichlorodifluoromethane (Halon 12), ethyl acetate, halothane, isobutane, isopropanol, isopropyl nitrate, methyl ethyl ketone, propane, tetrachloroethylene, toluene, 1,1,1-trichlorothane, 2,2,2-trichlorethanol, trichloroethylene and trichlorofluoromethane (Halon 11) in blood specimens obtained from patients suspected of abusing these agents.
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