Detection and identification of specific serum protein markers of non-small cell lung cancer

Abstract

Objective To detect the specific serum protein biomarkers for non-small cell lung cancer (NSCLC) by using proteomics techniques,and verify their detection efficacy for NSCLC.Methods Serum proteomic profiles of 112 cases of NSCLG and 123 controls were analyzed using surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI-TOF-MS),and the candidate protein biomarkers were identified by a series of proteomic methods and validated by ProteinChip immunoassays.Finally,the efficacy of these protein biomarkers for the detection of NSCLC was verified.Results These proteins with m/z of 6628,9191,11 412 could discriminated NSCLC from non-cancer controls with high detectable ability.The protein biomarker with m/z of 6628 was down-regulated in NSCLC patients and identified as apolipoprotein C-I,and the other two biomarkers with m/z of 9191 and 11 412 were up-regulated and identified as haptoglobin alpha-1 chain and S100A4,respectively.The sensitivity and specificity of this detective model were 96.56％ and 94.79％ respectively in the blind testing set.Conclusion Apolipoprotein C-I,haptoglobin alpha-1 chain and S10OA4 could be used for the early diagnosis of NSCLC as specific serum protein biomarkers. Key words: Apolipoprotein C-I; Haptoglobin alpha-1 chain; S100A4; Non-small cell lung cancer; Proteomics