Abstract
BackgroundDetection of Tuberculosis agent like nontuberculous mycobacteria (NTM) species by culture and microscopic methods remains difficult and time consuming. A fast and reliable diagnosis of tuberculosis would greatly improve the control of the disease. The purpose of this study is to compare the conventional multiplex PCR and multiplex PCR reverse cross blot hybridization assay to culture method in terms of mycobacteria species detection.FindingsAmong the 117 positively cultured samples, nontuberculous mycobacteria (NTM) species were found in 9 samples of multiplex PCR reverse cross blot hybridization assay; compared to only 3 NTM species found in our conventional multiplex PCR, and 13 NTM species were successfully identified among 162 negatively cultured samples compared to only 5 NTM species identification in conventional multiplex PCR results.ConclusionsThe sensitivity of the multiplex PCR reverse cross blot hybridization assay comparing to culture method was 86.03%, the specificity is 35.46%, the positive predictive value was 41.94% and the negative predictive value was 82.41%. For conventional multiplex PCR these values are 81.62%, 38.65%, 41.89%, 79.51%, respectively. Furthermore, in terms of mycobacteria species detection, the conventional multiplex PCR was relatively equal compared to the multiplex PCR reverse cross blot hybridization assay, and to be particularly having no significant discrepant results on the identification of Mycobacteria tuberculosis in both methods.
Highlights
Detection of Tuberculosis agent like nontuberculous mycobacteria (NTM) species by culture and microscopic methods remains difficult and time consuming
The sensitivity of the multiplex Polymerase Chain Reaction (PCR) reverse cross blot hybridization assay comparing to culture method was 86.03%, the specificity is 35.46%, the positive predictive value was 41.94% and the negative predictive value was 82.41%
In terms of mycobacteria species detection, the conventional multiplex PCR was relatively equal compared to the multiplex PCR reverse cross blot hybridization assay, and to be having no significant discrepant results on the identification of Mycobacteria tuberculosis in both methods
Summary
Detection of Tuberculosis agent like nontuberculous mycobacteria (NTM) species by culture and microscopic methods remains difficult and time consuming. The purpose of this study is to compare the conventional multiplex PCR and multiplex PCR reverse cross blot hybridization assay to culture method in terms of mycobacteria species detection. Tuberculosis is caused by Mycobacterium tuberculosis, Mycobacterium bovis or Mycobacterium africanum. Together with Mycobacterium microti and the vaccine strain M. bovis BCG they belong to the Mycobacterium tuberculosis complex. Some other cases caused by nontuberculous mycobacteria (NTM), are mostly mycobacteria belonging to the Mycobacterium avium-Mycobacterium intracellulare complex [1]. Opportunistic mycobacteria commonly associated with the human immunodeficiency virus (HIV) are Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium fortuitum and Mycobacterium scrofulaceum [1]. Mortality is highest in developing countries, where over three-quarters of cases occur [3]
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