Detection and identification of hybrids in the coenopopulation of co-growing species of Cypripedium (Orchidaceae) in the Novosibirsk Region

Elena Zhmud,Irina Kuban,Aleksandra Nabieva,Natasha Williams,Olga Dorogina,E.V Banaev,Y.G Zaytseva,M.A Tomoshevich

doi:10.1051/bioconf/20202400099

Abstract

Cypripedium calceolus L. and C. macranthon Sw. (Orchidaceae) are rare species throughout almost the entire territory of Russia. Though the data on intraspecific genetic differentiation has been presented in the literature, the species of C. calceolus, C. macranthon and alleged hybrids of C. × ventricosum growing in sympatric population in the Iskitim District of the Novosibirsk Region (NR) have not been investigated yet. The purpose of this work is dentification of the rare species of Cypripedium calceolus, C. macranthon and detection of C. × ventricosum hybrids in the NR using morphometric parameters and ISSR markers. It was found the mature generative specimens of C. × ventricosum formed the largest number of shoots, compared with the parent species. According to the number of flowers on the generative shoot, the hybrid occupied an intermediate position. According to the ISSR analysis, components from both parental species were identified in each of the three hybrids studied. Therefore, the presence of C. × ventricosum hybrid plants in the local NR coenopopulation of co-growing species C. calceolus and C. macranthon was detected and confirmed based on the study of morphometric parameters and ISSR markers.

Highlights

Almost throughout the whole territory of Russia, Cypripedium calceolus L. and C. macranthon Sw. (Orchidaceae) are rare species declining in number as a result of changes in the conditions of existence and destruction of habitats
In spite of the available data on the intraspecific genetic differentiation obtained using ISSR markers for the representatives of Cypripedium genus [7, 8], the species C. calceolus, C. macranthon and assumed hybrid С. × ventricosum growing in the Novosibirsk Region (NR) have not beens tudied yet
Representatives of C. × ventricosum differed by the high potential productivity, which was expressed as large clumps with a large number of shoots

Summary

Introduction

Almost throughout the whole territory of Russia, Cypripedium calceolus L. and C. macranthon Sw. (Orchidaceae) are rare species declining in number as a result of changes in the conditions of existence and destruction of habitats. (Orchidaceae) are rare species declining in number as a result of changes in the conditions of existence and destruction of habitats. These species are included in the Red Book of the Russian Federation with a status of 2a [1]. In spite of the available data on the intraspecific genetic differentiation obtained using ISSR markers for the representatives of Cypripedium genus [7, 8], the species C. calceolus, C. macranthon and assumed hybrid С. The purpose of this work is to use the morphometric parameters and ISSR markers to identify rare species of Cypripedium calceolus, C. macranthon and reveal their hybrids C. The purpose of this work is to use the morphometric parameters and ISSR markers to identify rare species of Cypripedium calceolus, C. macranthon and reveal their hybrids C. × ventricosum in the sympatric population

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Conclusion