Abstract
PurposeDevelop and evaluate an electrochemical method to identify healthy individuals, malignant hematopathic patients and solid tumor patients by detecting the leukocytes in whole-blood.MethodsA total of 114 individual blood samples obtained from our affiliated hospital in China (June 2015- August 2015) were divided into three groups: healthy individuals (n = 35), hematologic malignancies (n = 41) and solid tumors (n = 38). An electrochemical workstation system was used to measure differential pulse voltammetry due to the different electrochemical behaviors of leukocytes in blood samples. Then, one-way analysis of variance (ANOVA) was applied to analyze the scanning curves and to compare the peak potential and peak current.ResultsThe scanning curve demonstrated the specific electrochemical behaviors of the blank potassium ferricyanide solution and that mixed with blood samples in different groups. Significant differences in mean peak potentials of mixture and shifts (ΔEp (mV)) were observed of the three groups (P< = 0.001). 106.00±9.00 and 3.14±7.48 for Group healthy individuals, 120.90±11.18 and 18.10±8.81 for Group hematologic malignancies, 136.84±11.53 and 32.89±10.50 for Group solid tumors, respectively. In contrast, there were no significant differences in the peak currents and shifts.ConclusionsThe newly developed method to apply the electrochemical workstation system to identify hematologic malignancies and solid tumors with good sensitivity and specificity might be effective, suggesting a potential utility in clinical application.
Highlights
Hematologic malignancies have become a big threat to individuals of all ages and affected the living quality of people worldwide [1, 2]
The aim of this study was to develop and evaluate a method to identify hematologic malignancies and solid tumors by detecting the electrochemical characteristics of leukocytes in the peripheral blood of patients, which might be effectively used in clinical application
Reports have shown that the redox system in living cells and the electrode may be connected by proteins across the membrane system of the cells [33,34,35]
Summary
Hematologic malignancies have become a big threat to individuals of all ages and affected the living quality of people worldwide [1, 2]. Latest and suitable chemotherapy drugs on targeted tumors are important to improve the clinical outcomes of patients with hematologic malignancies, accurate early detection should be prioritized. Current methods and techniques in the diagnosis of hematologic malignancies include biopsy [3], peripheral blood testing [4], bone marrow biopsy [5], immunology testing [6], flow cytometry [7], radiologic examination[8], chromosome analysis and DNA sequencing technology [9], etc. Due to the disadvantages such as high cost, time consuming, complexity, and radioactive pollution of above mentioned clinical detection techniques [10], it is interest to develop a new user-friendly and cost-effective technology with good sensitivity to detect hematologic malignancies. The superior advantages of bio-electrochemistry applied in clinical diagnostics such as high sensitivity, portability and rapid detection have attracted attention of manyresearchers and physicians [11,12,13,14]
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