Abstract
Although acute gastroenteritis (AGE) has been reported as a common infectious disease in children, there is scarce information about enterovirus (EV) circulating associated with AGE cases in Brazil. The purpose of the present study was to identify and characterize the enteroviruses associated with AGE in children in Belém, Brazil. A total of 175 stool samples were obtained from children hospitalized revealing the presence of EV in 26.3% (46/175) of infections. EV type was identified in 78.3% (36/46) and EV-B species (61.1%; 22/36) was the most prevalent EV-detected followed by EV-C (25%; 9/36) and EV-A (13.9%; 5/36). This study has provided important information about the enterovirus circulation in Pará state, Northern Brazil.
Highlights
Acute gastroenteritis (AGE) has been reported as a common infectious disease in children, there is scarce information about enterovirus (EV) circulating associated with acute gastroenteritis (AGE) cases in Brazil
EV-positive samples (46/175; 26.3%) in the real time RT-PCR (rRT-PCR) were submitted to viral isolation in RD and HEp2-C cell lines and incubated at 37oC and examined daily for cytopathic effect (CPE) with total destruction of the cell monolayer, which is a characteristics of enterovirus infection
Noteworthy that the specimens were tested for other viral agents, such as rotavirus, parechovirus and aichivirus and the coinfection EV and non-enterovirus was observed with aichivirus
Summary
Acute gastroenteritis (AGE) has been reported as a common infectious disease in children, there is scarce information about enterovirus (EV) circulating associated with AGE cases in Brazil. *Correspondence: fernandotavares@iec.gov.br 1 Laboratório de Referência Regional em Enteroviroses, Seção de Virologia, Instituto Evandro Chagas, Ananindeua, Pará, Brasil Full list of author information is available at the end of the article with diarrhea in humans [8, 9]. The Picornaviridae family has the Enterovirus genus, whose association with AGE has been recognized and reported in many studies [1, 7, 10].
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