Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil.

Larissa Cristina Prado Das Neves Costa,Hugo Reis Resque,Jones Anderson Monteiro Siqueira,Yvone Benchimol Gabbay,Thayara Morais Portal,Alexandre Da Costa Linhares,Edivaldo Costa Sousa Júnior

doi:10.1590/0037-8682-0198-2017

Larissa Cristina Prado Das Neves Costa, Hugo Reis Resque + Show 5 more

Open Access

https://doi.org/10.1590/0037-8682-0198-2017

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Abstract

Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries. Human adenovirus (HAdV) and sapovirus (SaV) are among the agents that cause AGE. The present study aimed to detect and genotype HAdV and SaV in 172 fecal samples from children with AGE, collected during a surveillance study carried out in a low-income community in Belém, Pará, between 1990 and 1992. HAdV was detected by nested PCR, using primers Hex1deg/Hex2deg and NeHex3deg/NeHex4deg. SaV was assayed by reverse transcription PCR (RT-PCR), nested PCR, and quantitative PCR. The nucleotide sequence was determined by direct cycle sequencing. Overall, 43% (74/172) of samples were positive for HAdV, of which 70.3% (52/74) were sequenced and classified as belonging to five different species, mostly A and F. For SaV, positivity was 5.2% (9/172) and genotypes GI.1, GI.7, GII.1, and GV.2 were detected. The present results reinforce the need for further studies to obtain epidemiological data about the circulation of these viruses in Brazil, especially in the Amazon Region, where data from the early 1990's are scarce. Furthermore, the study describes for the first time the detection of SaV genotypes GI.7 and GV.2 in Brazil, showing that these types circulated in the region more than 25 years ago.

Highlights

Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries
The present study aimed to investigate the occurrence of Human adenovirus (HAdV) and SaV infections at the community level, utilizing a collection of fecal samples from children, who were followed up for acute gastroenteritis during 1990-1992 in Belém, Northern Brazil
Of the 3,075 samples collected throughout the study period, 1,990 were from diarrheic children, of which 4.6% (91/1,990) and 5.4% (34/624) were positive for rotavirus and astrovirus, respectively . 13,14 Of the remaining 1,865 rotavirus- andastrovirus-negative samples, we randomly selected a subset of 172 samples, which represented approximately 10% of the overall samples collected during each month

Summary

INTRODUCTION

Acute gastroenteritis (AGE) remains a serious global health problem, especially among children in developing countries. Sapovirus is an etiological agent of both outbreaks and sporadic cases of human AGE detected in all ages It belongs to the Caliciviridae family, has an icosahedral symmetry, is a non-enveloped virus, has a diameter of 27-40nm, and possesses a single-stranded ribonucleic acid (RNA) genome. With the advent of molecular techniques, it is possible to detect and genotype enteric viruses, and improve our knowledge of their molecular epidemiological profiles This is important in the Northern Region of Brazil, where data on the occurrence of such viruses are scarce. The present study aimed to investigate the occurrence of HAdV and SaV infections at the community level, utilizing a collection of fecal samples from children, who were followed up for acute gastroenteritis during 1990-1992 in Belém, Northern Brazil

METHODS

Ethical considerations

RESULTS

DISCUSSION