Abstract
Porcine Bocavirus (PBoV) has been reported to be associated with postweaning multisystemic wasting syndrome and pneumonia in pigs. In this study, a survey was conducted to evaluate the prevalence of PBoV in slaughter pigs, sick pigs, asymptomatic pigs and classical swine fever virus (CSFV) eradication plan herds in five provinces of China (Henan, Liaoning, Shandong, Hebei and Tianjin) by means of PCR targeting NS1 gene of PBoV. Among the total of 403 tissue samples, 11.41% were positive for PBoV. The positive rates of spleen (20.75%) and inguinal lymph node (27.18%) are higher than those of other organs. PCR products of twenty PBoV positive samples from slaughter pigs were sequenced for phylogenetic analysis. The result revealed that PBoV could be divided into 6 groups (PBoV-a~PBoV-f). All PBoV sequenced in this study belong to PBoV-a–PBoV-d with 90.1% to 99% nucleotide identities. Our results exhibited significant genetic diversity of PBoV and suggested a complex prevalence of PBoV in Chinese swine herds. Whether this diversity of PBoV has a significance to pig production or even public health remains to be further studied.
Highlights
Parvoviruses, members of the family Parvoviridae, are able to cause a broad spectrum of diseases in animals [1]
4 open reading frames (ORFs) have been identified: ORF1 at the 5 end encodes nonstructural protein NS1; ORF2 which is in the middle of the genome encodes nonstructural protein NP1, a unique Bocavirus characteristic structure that is absent in most other members of Parvoviridae [5]; overlapping ORF3 and ORF4 encodes structural proteins VP1 and VP2, respectively [5]
Porcine Bocavirus (PBoV1) based on partial VP1/2 genes was first discovered in postweaning multisystemic wasting syndrome (PMWS) affected pigs in Sweden [10], and the complete genome of PBoV types 1 and 2 was characterized and reported in 2010 [12, 13]
Summary
Parvoviruses, members of the family Parvoviridae, are able to cause a broad spectrum of diseases in animals [1]. Porcine Bocavirus (PBoV1) based on partial VP1/2 genes was first discovered in PMWS affected pigs in Sweden [10], and the complete genome of PBoV types 1 and 2 was characterized and reported in 2010 [12, 13]. Two novel PBoV types, 3 and 4, were discovered in Hong Kong based on the phylogenetic analysis of selected VP1 gene fragments [14]. A survey on the prevalence of PBoV in different parts of China was conducted by PCR detection on collected pig tissue samples with NS1 gene specific primers. Our observations could provide information for understanding the circulation and variety of PBoV in different herds or regions of China and for evaluating the role of PBoV in public health issue
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