Detection and functional assessment of complement receptors on two murine macrophage-like cell lines

Abstract

The complement receptors and associated activities of the murine macrophage-like cell lines P388D1 and IC-21 were compared with those of resident and thioglycolate-elicited peritoneal macrophages. Resident and P388D1 macrophages possessed receptors for both the C3b and C3d components of complement but failed to ingest particles bound to either of these receptor sites. Thioglycolate-elicited and IC-21 macrophages readily bound and ingested C3b particles but had reduced receptor and phagocytic activities when tested with C3d. These cell lines may represent distinct types of macrophages, each with an in vivo counterpart.