Abstract
A normal phase high performance thin layer chromatography (HPTLC) method has been developed and validated for simultaneous estimation of four components, namely, alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane from two medicinally important plants, Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. In Ayurveda, both plants have been reported to possess immunomodulatory activity. Chromatographic separation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. was performed on TLC aluminium plates precoated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at λ = 580 nm for α-amyrin, β-sitosterol, and lupeol, and at 366 nm for n-triacontane. The developed HPTLC method has been validated and used for simultaneous quantitation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. The developed HPTLC method is simple, rapid, and precise and can be used for routine quality control.
Highlights
Herbal medicines have been used since ages to treat various ailments
In the present research work, high performance thin layer chromatography (HPTLC) method has been developed as a quality control tool for whole plant powder of L. reticulata and P. lanceolata
The results showed that the peak areas of alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane almost remained unchanged over a period of 48 hrs, and no significant degradation was observed within the given period
Summary
Herbal medicines have been used since ages to treat various ailments. Ayurveda is an Indian traditional system of medicine used since ancient times. Some of the analytical methods for qualitative analysis of alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane from other plant samples are discussed . RP-HPTLC separation of twelve compounds including alpha-amyrin, lupeol, and beta-sitosterol from Brassica oleracea L., Solanum lycopersicum L., Rosmarinus officinalis L., Salvia officinalis L., and Quercus robur L. was carried out [9]. No method was applied for quantifying the presence of alpha-amyrin, beta-sitosterol, lupeol, and ntriacontane simultaneously from L. reticulata and P. lanceolata. In present research work, a simple, rapid, precise, and accurate HPTLC method has been developed and validated using International Conference on Harmonization (ICH) guidelines for simultaneous determination and quantification of alpha-amyrin, beta-sitosterol, lupeol, and ntriacontane form dried whole plant powder of L. reticulata. In present research work, a simple, rapid, precise, and accurate HPTLC method has been developed and validated using International Conference on Harmonization (ICH) guidelines for simultaneous determination and quantification of alpha-amyrin, beta-sitosterol, lupeol, and ntriacontane form dried whole plant powder of L. reticulata. and P. lanceolata
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