Abstract
A systematic study has been made of an immunofluorescence technique for the kinetic demonstration of estradiol-17β in target cells. An experimental system suited for monitoring the steroid dynamics in cell suspensions has been developed. Pertinent control tests of the receptor binding and of the immune specificity have been carried out. Fluorescent antibody was able to specifically detect estradiol bound to receptors in various cell compartments (cytoplasm, nuclear chromatin and nucleolus) at different times and temperatures of incubation. Lack of cytoplasmic receptors and some defects of the two-step mechanism could also be demonstrated in mixed cell subpopulations in human breast cancer.
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