Detection and distribution of intestinal calcium-binding protein in the young adult male guinea pig.

Abstract

A calcium-binding factor was detected in guinea pig intestinal mucosa. It was negatively charged and moved in an electrical field. Pronase quickly destroyed almost completely the calcium-binding activity although trypsin had practically no effect. The major calcium-binding peak was well defined. It was concluded that the factor is probably a protein. Calcium-binding protein was found throughout the gastrointestinal tract, with the calcium-binding activity decreasing with increasing distance from the stomach.