Abstract
Specific oligonucleotide primers were synthesized using sequence data of the 16S rRNA gene from three plant-pathogenic mycoplasmalike organism (MLO) groups (I-III). When used as primers in the polymerase chain reaction (PCR), four sets of primer pairs were identified: 1) mollicute-specific, 2) MLO-specific, 3) MLO-group 1-specific, and 4) MLO-group III-specific. MLO 16S rRNA genes were selectively amplified by PCR from infected plants and vector insects. Using these primers and thermal cycling conditions, this technique was effective for detecting MLO 16S rRNA genes from diseased plants and infected insect vectors and for differentiating MLO 16S rRNA genes from similar rRNA genes of eukaryotic host organelles and other microorganisms associated with healthy vector insects [...]
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