Abstract
Chromatographic conditions were optimized and performance characteristics were evaluated for a procedure for the detection and determination of 12 phenolic and cinnamic acids in plant extracts by HPLC with spectrophotometric detection. Chromatographic separation was performed in the gradient elution mode, using an aqueous solution of trifluoroacetic acid and acetonitrile as a mobile phase. Sensitivity was increased by performing spectrophotometric detection at the absorbance maxima of individual acids. The procedure was used for the detection and determination of phenolic content in dried extracts from Astragalus dolichophyllus and Astragalus brachylobus.
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