Abstract
Detection and classification of soft rot Erwinia of vegetables in Thailand were conducted from ten varieties of vegetables; cauliflower, cabbage, chinese cabbage, pak-choi, spinach, carrot, chinese radish, cucumber, tomato and asparagus. To determine soft rot Erwinia, soft rot bacteria were isolated from rot symptom of these vegetables on pectate assay medium and identified by technique of DNA polymerase chain reaction. Using Y1 and Y2 primers are specific to amplify 434 bp open reading frames of pectate lyase (pel) genes of E. carotovora while ADE1 and ADE2 primers are specific to amplify 420 bp open reading frames of pectate lyase genes of E. chrysanthemi. The result showed that only Y1 and Y2 primers were able to amplify genomic DNA of all isolates. [...]
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