Abstract
Sixteen ivy gourd ( Coccinia grandis (L.) Voigt) plant samples showing severe mosaic symptoms were collected from New Delhi and Varanasi (Uttar Pradesh) in India. Begomovirus infection was confirmed by PCR using begomovirus-specific primers. Amplified PCR products (1.2 kb fragments) were cloned and the sequence was characterized. Based on sequence analysis, begomovirus associated with the majority of ivy gourd samples (16) was found to be a member of a bipartite begomovirus species, which is closely related to tomato leaf curl New Delhi virus (ToLCNDV). Therefore, two samples of ivy gourd, IVG1-ND and IVG2-Var, were selected for full-length genome (DNA-A and DNA-B-like sequence) amplification by the rolling circle DNA amplification (RCA) method. Sequence analysis performed using the Species Demarcation Tool (SDT) program revealed that they share 89.5-91.3% (IVG1-ND) and 93.4-96.8% (IVG2-Var) nucleotide (nt) identity with the DNA-A-like sequence of ToLCNDV isolated from cucurbits and chilli, respectively. The IVG1-ND and IVG2-Var isolates shared 90% nt identity among themselves, indicating that they are two different strains of ToLCNDV. Similarly, SDT analysis of the DNA-B-like sequence of IVG1-ND and IVG2-Var exhibited showed 82.7-93.3% nt identity with the DNA-B-like sequences of ToLCNDV infecting cucurbits. The recombination analysis of DNA-A and DNB-B-like sequences showed that the greater part of their genome most likely originated from previously reported begomoviruses that are known to infect chilli and cucurbits through recombination. https://doi.org/10.2298/ABS170616051V Received: June 16, 2017; Revised: November 16, 2017; Accepted: November 29, 2017; Published online: December 21, 2017 How to cite this article: Venkataravanappa V, Narasimha Reddy LRC, Saha S, Subbanna SK, Manem KR. Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd ( Coccinia grandis (L.) Voigt) in North India. Arch Biol Sci. 2018;70(2):339-47.
Highlights
Ivy gourd (Coccinia grandis (L.) Voigt) is an important vegetable and medicinal plant from the Cucurbitaceae family
The begomoviruses transmitted by Bemisia tabaci are becoming the major constraint in the production of many crops belonging to different taxonomic classes
The detection of tomato leaf curl New Delhi virus (ToLCNDV) in the ivy gourd samples collected from Varanasi, Mirajpur and New Delhi, and their subsequent genome sequencing and analysis in the current study adds it to the list of its expanding host range
Summary
Ivy gourd (Coccinia grandis (L.) Voigt) is an important vegetable and medicinal plant from the Cucurbitaceae family. It is distributed in tropical Asia, Africa, Pakistan, India and Sri Lanka [1,2]. Detection and characterization of tomato leaf curl New. Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India. Arch Biol Sci. 2018;70(2):339 virus [10], ageratum enation virus [11] and Coccinia mosaic virus [12] Among these viruses, ToLCNDV has become a major limiting factor for the production of many crop plants [13]. DNA-B contains the BV1 gene in the sense strand, and the BC1 gene in the complementary strand [14]
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