Abstract
Rubus spp. plants with severe symptoms of stunting, short and thin shoots were observed in central Poland in both production fields (raspberry and blackberry) and their natural environments. Nested PCR of DNA extracted from symptomatic plants and healthy raspberry, conducted using phytoplasma universal and groupspecific primer pairs, showed the presence of phytoplasmas in all symptom-showing plants selected for this study. RFLP with HpaII, RsaI, HhaI and BfaI and sequence analysis of the 16S rDNA fragment amplified with universal primers R16F2n/R16R2 revealed that assayed plants were infected by phytoplasmas belonging to three different groups. This is the first report of the natural occurrence of ‘Candidatus Phytoplasma asteris’ and X disease phytoplasma in Rubus spp. in Poland.
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