Abstract

The aim of this study was to isolate the antimicrobial-resistant enterobacteria from cattle feces and to identify the extended-spectrum beta-lactamase (ESBL) and carbapenemase gene among them. A total of 70 cattle fecal swab samples were collected from Durg, Chhattisgarh, India, and cultured in nutrient broth. Bacterial isolation recovered 120 gram-negative bacteria. Antimicrobial susceptibility testing showed 90 (75%) coliform isolates (66 Escherichia coli , 20 Klebsiella sp., and two each of Enterobacter sp. and Citrobacter sp.) resistant to amoxicillin, amoxicillin-clavulanic acid, cefixime, ceftazidime, imipenem, or meropenem. Bacterial isolates showed the highest resistance rate for ceftazidime (61.6%), followed by cefixime and amoxicillin-clavulanic acid (60%). The ESBL phenotype, assessed by combination disk method, was shown by 36 (40%) of the resistant isolates. PCR amplification revealed 20% and 31.6% positivity of bla T E M role=presentation> T E M T E M _{TEM} and bla S H V role=presentation> S H V S H V _{SHV} genes, respectively. bla T E M role=presentation> T E M T E M _{TEM} and bla S H V role=presentation> S H V S H V _{SHV} coexpression was recorded among eight (12.1%) E. coli and two (10%) Klebsiella sp. isolates. Restriction endonuclease analysis indicated clonal blaTEM and clonal and nonclonal blaSHV genes. Nucleotide sequencing confirmed the bla T E M role=presentation> T E M T E M _{TEM} and bla S H V role=presentation> S H V S H V _{SHV} genotypes. None of the isolates showed amplification of the carbapenemase gene. Phenotypic and genetic detection and characterization of ESBLs among Enterobacteriaceae bacteria illustrate the importance of planning and execution of effective control programs for emerging antimicrobial-resistant pathogens.

Highlights

  • One of the most important mechanisms for development of antimicrobial resistance of bacteria is mediated by the production of bacterial beta-lactamases

  • The Enterobacteriaceae are inhabitants of the alimentary tracts of animals and humans and are shed along with the feces into the environment, where they may act as potential threats to public health

  • Beta-lactam resistance in the present study was recorded among the isolates of the coliform group (Escherichia coli, Klebsiella, Enterobacter, and Citrobacter) of Enterobacteriaceae

Read more

Summary

Introduction

One of the most important mechanisms for development of antimicrobial resistance of bacteria is mediated by the production of bacterial beta-lactamases. Extendedspectrum beta-lactamases (ESBLs) are capable of targeting a broader spectrum of antibiotics, including penicillins and their complexes, monobactams, and up to third-generation cephalosporins [1]. Members of Enterobacteriaceae are commensals in the intestines of animals and are usually under selective pressure by use of antibiotics for treatment and prevention of infectious diseases [2]. Escherichia coli and Klebsiella pneumoniae expressing ESBLs in particular are important microbial pathogens that have been reported more frequently in association with the acquisition and transmission of drug-resistance elements [3,4]. RAWAT et al / Turk J Vet Anim Sci

Objectives
Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call