Abstract
A bulk acoustic wave ammonia sensor has been applied to monitor the growth of Proteus mirabilis based on the growth response equation. The influence of temperature on the bacterial growth has been investigated by using the Arrhenius law. A temperature-dependent model, called the Schoolfield model, has also been used to fit the maximum specific growth rates ( μ m) at different temperature. The goodness-of-fit is 1.0007 and the characteristics parameters of Proteus mirabili in the Schoolfield model have been estimated. The growth rate at 25°C ( ϱ (298 K)), the activation enthalpyy ofthe rate-controlling enzyme reaction, the optimum growth temperature ( T opt) and the optimum specific growth rate ( μ m opt) are 1.023 (h −1), 7 (kJ mol −1), 312.4 K and 1.3364 (h −1), respectively. In addition, the clinical assay of bacterial concentration in human urine samples has been accomplished, based on a linear relationship between the inflection time ( t i) and the logarithm of the bacterial concentration. Results obtained for the bacterial concentration are in agreement with those obtained by the pour plate count method and the frequency detection time method respectively.
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