Abstract
The stacked-trait transgenic crop is a novel transgenic crop obtained by traditional cross-breeding of single-trait transgenic crops. In this study, real-time fluorescent quantitative PCR method was used for detection and analysis of stacked-trait transgenic events in 7 batches of imported practical soybean samples. Eight transgenic soybean events (GTS40-3-2, DAS44406-6, A5547-127, MON89788, MON87708, A2704-12, MON87701 and FG72) were identified. Single-seed and multi-target detection were further applied. Fifteen practical soybean seeds were extracted randomly from each batch and the stacked-trait MON87708 × MON89788 event was detected in 6 batches of samples. Detection rates were 20%, 73.3%, 86.7%, 20%, 86.6% and 40% with relative estimation contents of 7.58%, 55.46%, 70.70%, 41.73%, 49.63% and 16.14%, respectively. This research provided reference and guidance criteria for detection and supervision of imported transgenic soybean events.
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