Detection and analysis of copy number aberration of somatic mutation in hepatocellular carcinoma based on genome-wide next generation sequencing

Abstract

Objective To detect and analyze the genome-wide distribution of the copy number aberrations (CNAs) of somatic mutation in hepatocellular carcinoma, and to investigate the susceptibility loci and related genes of somatic mutation. Methods Illumina Hiseq 2500 sequencing platform was used to detect the genome-wide sequence of tumor tissues and adjacent tissues in 1 patient with hepatocellular carcinoma after the operation. Bioinformatics analysis was performed on CNAs of somatic mutation in the tumor tissues. Results The average sequencing depth and coverage of the adjacent tissues and tumor tissues were 12.31, 90.24% and 11.37, 92.48%, respectively. The three peaks of CNAs in somatic mutations were located at 10q26.13, 17p13.1 and 22q13.2, respectively. In the above three loci, the number of CNAs containing the exons of the coding gene were 7, 3 and 7, respectively. Conclusion The somatic mutation loci are different from the susceptible loci, which will provide the basis for the mechanism of hepatocellular carcinoma caused by environmental risk factors induced somatic mutation. Key words: Carcinoma, hepatocellular; Somatic mutation; Copy number aberration; Genome