Detection and Analysis of Autoantigens on the Membrane of Bone Marrow Cells In the Patients with BMMNC- Coomb's Test(+) Hemocytopenia

Abstract

AbstractAbstract 4434 ObjectiveTo investigate the autoantigens on the membrane of bone marrow cells in the patients with bone marrow mononuclear cell Coomb's(BMMNC-Coomb's) test(+) Immuno-related pancytopenia(IRP),for further purification and cloning. Methods20 newly diagnosed IRP patients, 10 AA patients, 10 MDS patients and 10 healthy donors as controls were enrolled in this study.The level of autoantibody-IgG in bone marrow was measured by Western blot; The autoantigens targeted by autoantibody IgG in IRP were identified from membrane protein extracts by SDS-PAGE and Western blot. ResultsThe level of autoantibody IgG in bone marrow of untreated IRP patients was 75% (15/20), which was significantly higher than that of AA or MDS patients (0%) and normal controls(10%)(P<0.01). Autoantibody IgG in IRP could react with several autoantigens with approximate MWs of 25–30kDa, 60–65 kDa, 73 kDa and 83 kDa. Positive rates of individual autoantigens were 35%,50%,20% and 10%,respectively. ConclusionAutoantibody IgG reacted with membrane antigens could be found in bone marrow in IRP patients. The molecular weights of the autoantigens were mainly 25–30kDa, 60–65 kDa, 73 kDa and 83 kDa. The result showed that autoantibody IgG played an important role in IRP. Disclosures:No relevant conflicts of interest to declare.