Abstract
Tomato (Lycopersicon esculentum Mill) is edible, often red berry-type fruit and a rich source of Fe, Vitamin A, B and C. It is widely cultivated all over the world. E8 gene is related to ethylene biosynthesis in plants. Ethylene is responsible for growth and ripening of fruits. The present study was carried out to detect and amplify the E8 Promoter in the leaves and fruits of Tomato, Spring onion, Capsicum and Lettuce. The CTAB Protocol of Doyle and Doyle (1990) were used for DNA extraction from leaves and fruits and quality was checked on gel electrophoresis. PCR program for E8 was optimized and PCR products were sequenced. The sequencing results were aligned and Blast. The result indicated that in Tomato fruit the E8 gene is detected and amplified as compare to leaves and other plants. This E8 primer will be utilized for different fruits as well.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
