Detecting the Protein (Pns10) Encoded by Genome Segment 10 of Rice Ragged Stunt Oryzavirus in Diseased Rice and Viruliferous Vector Insects.

Abstract

The rice ragged stunt virus genome contains 10 dsRNA segments. In segment 10 (S10), a minicistron (nt 20-55) precedes the major ORF (nt 142-1032). The initiation codon of the minicistron was recognized by in vitro translation of wheat germ extracts, while the product of the major downstream ORF was not detected. In order to investigate the expression of the major ORF in vivo, Pns10 was expressed in bacteria and antiserum was produced for immunodetection. Although the detected protein migrated slightly slower on SDS-PAGE than that expressed by bacteria, the major S10 ORF was expressed as a 34kDa protein in both diseased rice plants and viruliferous vector insects.