Abstract
Genomic in situ hybridization (GISH), using genomic DNA probe from O. australiensis, was used to study chromosome pairing among AA, EE and AE genomes, in the hybrid O. sativa×O. australiensis. In the conventional cytogenetic analysis, 0–4 bivalents and 20–24 univalents were recorded. GISH, however, revealed 1–5 bivalents and 19–23 univalents. 3 types of pairing were detected: pairing between A and E genome chromosomes, within AA genome chromosomes and within EE genome chromosomes. The frequency of association between O. sativa (AA) and O. australiensis (EE) chromosomes (0.98II/cell) greatly exceeded the level of pairing, within sativa chromosomes (0.15II/cell) or within australiensis chromosomes (0.05II/cell). Results indicated that conventional cytogenetic analysis either underestimates or overestimates the pairing behavior and that GISH is a powerful tool for detecting the nature of pairing in O. sativa×O. australiensis.
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