Abstract
The binding of radiolabeled reagents to cells is detected by coating the specimen with a photographic emulsion. When the emulsion is developed, silver grains can be seen at the location of the radiolabel. The silver grains appear as black dots in bright-field microscopy but are most readily detected by dark-field illumination, in which they are seen as intense silver reflections on a black background. The detection of iodine-labeled reagents is extremely sensitive, and the reaction is readily controlled by setting up duplicate samples and varying the exposure time. The method is compatible with histological stains and can be used in double-labeling procedures with enzyme- or fluorochrome-labeled reagents. When iodine-labeled and enzyme-labeled reagents are used together for double labeling, the enzyme-labeled reagents must be developed before the photographic emulsion is added.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
