Abstract
DETECHIP® is a detection system made of various sensors that has been shown to detect and discriminate between small molecules of interest, including various illicit and over-the-counter drugs. Previously, detection was normalized to a single concentration of analyte. Now this detection assay can detect concentration differences in analytes via red, green, and blue color value changes and shifts in the UV-Vis spectra of the assay. To determine the concentrations differences, the exposed assays were scanned on a flatbed scanner and the images were analyzed for individual RGB values with a custom macro in ImageJ, an image analysis program. Increasing concentrations of the analyte resulted in greater differences in color values between control and analyte wells. These differences showed a linear relationship to concentration change, some with correlation coefficients greater than 98%. This work expands the capability of DETECHIP to give information about the concentration of analyte when the analyte identity is known.
Highlights
DETECHIP, short for detection chip, is a developing technology containing molecular sensors DC1-8 which discriminate between analytes via differential interactions with analytes resulting in colorimetric changes [1]
DETECHIP molecular sensors were examined by RGB image analysis and UV-vis spectroscopy to determine if concentration changes can be detected
For each concentration of ketamine tested from 10 mM to 100 mM, an identifying code was generated as shown in Table 1, with the unique identifying RGB code differing for each concentration
Summary
DETECHIP , short for detection chip, is a developing technology containing molecular sensors DC1-8 which discriminate between analytes via differential interactions with analytes resulting in colorimetric changes [1]. The molecular structures of molecular sensors DC1-DC8 are shown in Figure 1 [2,3]. This detection technique is a simple assay that has been proven effective in detection of explosives in the field, performance-enhancing drugs in competitive sports, abused narcotics, and other small molecules of interest [4]. Colorimetric changes in DETECHIP are measured with computer analysis of assay images that is able to quantify red, green, and blue (RGB) color values, or by examination of UV-vis spectroscopy of control and analyte-treated solutions [4,5,6]. DETECHIP molecular sensors were examined by RGB image analysis and UV-vis spectroscopy to determine if concentration changes can be detected. DETECHIP detection of analyte concentration could provide an alternative to costly, time-consuming methods and expands the capabilities of this detection technique
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