Detecting a novel Eriocheir sinensis reovirus by reverse transcription loop-mediated isothermal amplification assay.

Abstract

Tremor disease (TD) is one of the most serious diseases of Eriocheir sinensis. A novel E.sinensis reovirus (EsRV) was identified from E.sinensis afflicted with TD and caused high mortality. We developed a reverse transcription loop-mediated isothermal amplification assay with high specificity, sensitivity and rapidity to detect EsRV, which can be used to diagnose aquatic animal diseases, particularly where expensive diagnostic instruments are not available.