Detect Structure Features of Asymmetric and Symmetric CH2 and CH3 Functional Groups and Their Ratio of Biopolymers within Intact Tissue in Complex Plant System Using Synchrotron FTIRM and DRIFT Molecular Spectroscopy

Abstract

1.1 Bright synchrotron radiation Synchrotron light is photon beam, which is million times brighter than sunlight. A synchrotron facility is a giant particle accelerator that turns electrons into light (Wikipedia, 2010). How a Synchrotron Works? Electric Gun (EG: Synchrotron Component 1) in a synchrotron blasts out electrons. The electrons are accelerated to nearly speed of light by Linear Accelerator (LA: Synchrotron Component 2). A small synchrotron orbiting Booster Ring (BR: Synchrotron Component 3) increase their energy. Finally electron bunches enter Storage Ring (SR: Synchrotron Component 4). The bunches orbit ring controlled by serial magnets. Special magnet called “Undulator causes electrons to rapidly change course and vast emitting energy in form of photons. Then photons enter Beamline (BL: Synchrotron Component 5) and directed to device called monochromator which selects specific wavelength required for each experiment. Finally, light focus onto sample at Experimental End Station (ES: Synchrotron Component 6), where scientists collect data to determine molecular structure (Dumas, 2003; Marinkovic et al., 2002; Miller, 2009; CLS, 2010; Wikipedia, 2010). The extremely bright synchrotron light makes it possible to detect biomaterial structure (make-up or conformation) at both molecular and cellular levels (Marinkovic & Chance, 2006; Miller & Dumas, 2006).