Abstract
Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions.To determine the B19 DNA carrier frequency in a population of volunteer blood donors from three hospitals blood banks in Santiago, Chile, and to determine the viral load in DNA positive cases.A total of 477 serum samples were analyzed. The screening of B19 DNA was carried out by nested polymerase chain reaction (PCR) directed to the non-structural region of the virus (NS1). The viral load in positives cases was quantified by NS1 Real Time PCR.Parvovirus B19 was detected in four samples, rendering a frequency of 1:119. The viral loads ranged from less than 2000 to 5626 x 10(5) genome equivalents/ml.Parvovirus B19 was present in four of 477 blood bank blood donors from three hospitals in Santiago.
Highlights
Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host
The viral load in positives cases was quantified by no estructural 1 (NS1) Real Time polymerase chain reaction (PCR)
Parvovirus B19 was detected in four samples, rendering a frequency of 1:119
Summary
Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. Lévican et al Estudios de prevalencia en donantes de sangre han detectado la presencia de ADN viral en amplios rangos, los que fluctúan entre 1 en 167 y 1 en 35.000, dependiendo de la situación epidemiológica y de la técnica de detección empleada. Estudios de seroprevalencia muestran que 60% de individuos en edad adulta temprana presentan anticuerpos IgG para este virus, lo que implica una alta tasa de exposición a la infección por B19 en estas edades[12]. El propósito de este trabajo es demostrar la presencia de ADN de B19 en donantes voluntarios de sangre en tres bancos de sangre de hospitales de la ciudad de Santiago, y a la vez determinar la magnitud de la viremia en los individuos que resulten positivos
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