Abstract
Abstract The use of bicarbonate additions to nutrient solutions containing low levels of available Fe have allowed screening for susceptibility to Fe‐deficiency chlorosis in many dicots (soybean, bean, chickpea, citrus, etc.). Research has shown that relative susceptibility among cultivars in this nutrient solution has a high correlation with the relative susceptibility observed in wet calcareous soils which induce chlorosis. This paper provided the specific details needed by plant breeders to adapt the research reports to their own laboratory situation. We provide full details on the present recommended method using FeDTPA (ferric diethylenetriaminepentaacetic acid) in place of FeEDDHA (ferric ethylene‐di‐o‐Chydroxyphenylacetic acid) used in earlier screens, including preparation of nutrient solutions, inducing varied severity of Fe‐stress or potential chlorosis severity, use of indicator cultivare, and measuring plant response. The method described has been developed in cooperation with industrial and a...
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