Detailed and banding karyotype analyses of the medaka, Oryzias latipes in cultured cells.

Abstract

Fin-culture was achieved in the medaka, Oryzias latipes, and detailed karyotype analyses were undertaken by differential C-banding and silver staining techniques. Wild and orange-redvarieties of the medaka showed the same karyotype characterized by 48 chromosomes consisting of 2 pairs of metacentrics, 8 pairs of submetacentrics, 1 pair of subtelocentrics, 13 pairs of acrocentrics (FN=68). One pair of the smaller submetacentrics showed stalks on their short arms with the appearance of the satellited chromosomes. The latter chromosomes also had NORs on their telomeric or stalk regions of the short arms. No characteristic C-banded chromosome was detected. Centromere regions of almost all chromosomes were C-band-positive. No sexual difference of the chromosomes was detected in the present study.