Detail work on formulation development and evaluation of micro sponges gel of clotrimazole for treatment of vaginal fungal infection

Abstract

Vaginal infection is so widespread that women have to seek medical counseling. In fact, almost 70% of women experience vaginal infections in their life. Vulvovaginal candidiasis is responsible for vaginal infections and Candida albicans is the major agent. Vaginal infections are caused by hormonal changes, negative sexual effects, irrelevant quality of life, high mortality rate, depressive mood and various kind of anxiety.Detail Work on Formulation Development and Evaluation of Micro sponges gel of Clotrimazole for Treatment of Vaginal fungal Infection. The microsponge-based novel delivery system has been developed for vaginal delivery of Clotrimazole. The method adopted was quasi-emulsion solvent diffusion. Formed microsponges were a spherical in shape Different drug–polymer ratio reflected good particle size, drug content and entrapment efficiency. Microsponge-based gel showed in vitro drug release reflected highest regression value for Koshmeyer-Peppas and in vitro antifungal activity of CLZ microsponges gel was higher than the market formulation.Drug, chemical and solvent to be used are listed as Clotrimazole, Eudragit RL-100, Polyvinyl alcohol, Carbopol 934, Sodium Citrate dihydrate, Citric Acid, Dichloromethane, Triethanolamine, Agar 1. Preparation of citrate buffer pH 4.5 solution 2. Preparation of standard curve of citrate buffer pH 4.5 solution: methanol 3. FTIR spectroscopy 4. Selection of method for the preparation of microsponges 5. Quasi emulsion solvent diffusion (two step method) is selected for the preparation of microsponges 6. Selection of amount of drug (clotrimazole): 7. Selection of optimum volume of solvent (dichloromethane): 8. Selection of amount of emulsifier (polyvinyl alcohol): 9. Preparation of microsponges.The f23 formulation, which had a manufacturing yield of 66.58%, an entrapment efficiency of 91.26%, and an actual drug content of 67.28 percent, was determined to be more trustworthy than the other formulations. The CDR was 66.18%, and the Flux value was 76.17(g/cm2/h).With (r2)0.9738 and a n value of 0.3981, Koshmeyer Peppas was judged to be the model that suited the data the best.A measurement of the viscosity revealed non-Newtonian flow. The ZOI, which exceeded the advertised preparation, was discovered to be 2.2 cm.For vaginal delivery of Clotrimazole, a new delivery mechanism based on microsponges has been created. The technique used was solvent diffusion from a quasi-emulsion. Microsponges that had been formed had a spherical shape. Different drug-polymer ratios were indicative of good drug content, entrapment effectiveness, and particle size. Microsponge-based gel demonstrated in vitro drug release that had the greatest regression value for Koshmeyer-Peppas, and CLZ microsponges gel had stronger in vitro antifungal activity than the commercial formulation.