Abstract
Several parameters that influence the dibenzothiophene (DBT) desulfurization by lyophilized cells of Pseudomonas delafieldii R-8 were studied in the presence of dodecane. The aqueous media tested with pH range in 4.6–8.5 made no obvious difference on the desulfurization activity. The rate and extent of desulfurization were strongly dependent on the volume ratio of oil-to-water, DBT concentration and the cell concentration. The specific desulfurization rate of DBT and 4,6-dimethyl DBT (4,6-DMDBT) could reach 11.4 and 9.4 mmol sulfur kg −1 dry cells (DCW) h −1, respectively. The desulfurization pattern of DBT was represented by the Michaelis–Menten equation. The kinetic parameters, the limiting maximal velocity ( V max) and Michaelis constant ( K m), for desulfurization of DBT were calculated.
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