Abstract
Background. To obtain reliable results of laboratory studies on the identification of Listeria, the presence of certified diagnostic agglutinating Listeria sera is required. An important step in the manufacturing process of such medical devices for in vitro diagnostics requires effective nutrient media for the accumulation of listeriosis microbe. Aim of the research. To develop an effective nutrient medium for the accumulation of bacterial mass of Listeria. Materials and methods. The object of the study was an experimental culture medium for Listeria cultivation. As a control, we used nutrient agar for the cultivation of microorganisms (fish meal hydrolysate, FMH-agar) and meat-peptone agar with 1 % glucose (MPA with 1 % glucose). The specific activity of nutrient media during cultivation of the test strain Listeria monocytogenes 766 was evaluated using a complex of microbiological methods. Results. The optimal base of the nutrient medium for Listeria cultivation has been selected: pancreatic hydrolysate of river magpie fish (Rutilus rutilus lacustris) and hydrolysate of meat water production waste. The qualitative and quantitative composition of the nutrient medium has been developed, its physical, chemical and biological properties have been studied. It was found that after 24 hours of incubation at 37 °C, the nutrient medium provided the growth of typical Listeria colonies. The germination rate was 85 %, which is higher compared to the growth of the culture on MPA with 1 % glucose and GRM agar by an average of 21 % (p < 0,05). Conclusion. The experimental culture medium for Listeria cultivation provided growth of colonies of the test strain L. monocytogenes 766 with the preservation of characteristic cultural, morphological and biochemical properties, and, in terms of germination and growth rate, exceeded the control media. The developed nutrient medium provides effective growth of Listeria and can be used as a medium for the accumulation of microbial mass.
Highlights
To obtain reliable results of laboratory studies on the identification of Listeria, the presence of certified diagnostic agglutinating Listeria sera is required
It was found that after 24 hours of incubation at 37 °C, the nutrient medium provided the growth of typical Listeria colonies
The germination rate was 85 %, which is higher compared to the growth of the culture on MPA with 1 % glucose and GRM agar by an average of 21 % (p < 0,05)
Summary
Хаптанова Н.М., Лукьянова С.В., Кузнецов В.И., Гефан Н.Г., Андреевская Н.М., Коновалова Ж.А., Остяк А.С., Косилко В.С. Цель исследования: разработка эффективной питательной среды для культивирования и накопления бактериальной массы листерий. Объектом исследования служила экспериментальная питательная среда для культивирования листерий сухая. Подобрана оптимальная основа питательной среды для культивирования листерий – панкреатический гидролизат речной рыбы сороги При изучении биологических свойств экспериментальной среды установлено, что через 24 часа инкубации при температуре 37 ± 1 °С питательная среда обеспечивала рост типичных колоний листерий. Экспериментальная питательная среда для культивирования листерий сухая обеспечивала рост колоний тест-штамма L. monocytogenes 766 с сохранением характерных культуральных, морфологических и биохимических свойств, а по показателям роста превосходила контрольные среды. Для цитирования: Хаптанова Н.М., Лукьянова С.В., Кузнецов В.И., Гефан Н.Г., Андреевская Н.М., Коновалова Ж.А., Остяк А.С., Косилко В.С.
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