Abstract
In order to obtain gold electrode surfaces modified with Human Papillomavirus L1 protein (HPV L1)-derived peptides, two sequences, SPINNTKPHEAR and YIK, were chosen. Both have been recognized by means of sera from patients infected with HPV. The molecules, Fc-Ahx-SPINNTKPHEAR, Ac–C–Ahx-(Fc)KSPINNTKPHEAR, Ac–C–Ahx-SPINNTKPHEAR(Fc)K, C–Ahx–SPINNTKPHEAR, and (YIK)2–Ahx–C, were designed, synthesized, and characterized. Our results suggest that peptides derived from the SPINNTKPHEAR sequence, containing ferrocene and cysteine residues, are not stable and not adequate for electrode surface modification. The surface of polycrystalline gold electrodes was modified with the peptides C-Ahx-SPINNTKPHEAR or (YIK)2-Ahx-C through self-assembly. The modified polycrystalline gold electrodes were characterized via infrared spectroscopy and electrochemical measurements. The thermodynamic parameters, surface coverage factor, and medium pH effect were determined for these surfaces. The results indicate that surface modification depends on the peptide sequence (length, amino acid composition, polyvalence, etc.). The influence of antipeptide antibodies on the voltammetric response of the modified electrode was evaluated by comparing results obtained with pre-immune and post-immune serum samples.
Highlights
According to the WHO (World Health Organization), cervical cancer is the second most common cancer among women worldwide
We report the design, synthesis and electrochemical study of self-assembled monolayers of two synthetic peptides derived from the Human Papillomavirus (HPV)-L1 protein whose original sequences are recognized by antibodies against HPV-16 [25,26]
Our approach was to couple antigenic peptides derived from HPV proteins to an electrode surface
Summary
According to the WHO (World Health Organization), cervical cancer is the second most common cancer among women worldwide. The electrochemical behavior of the modified electrode was examined via cyclic voltammetry in order to evaluate the redox behavior of the ferrocene moiety Changes in such behavior upon addition of the serum samples suggested that the fabricated bioelectrochemical sensor was able to recognize the interaction between antipeptide antibodies and the immobilized HPV-1 peptide with high selectivity and sensitivity. We report the design, synthesis and electrochemical study of self-assembled monolayers of two synthetic peptides derived from the HPV-L1 protein whose original sequences are recognized by antibodies against HPV-16 [25,26] These peptides, HPV-3 and HPV-4, have one cysteine residue at the C-terminal or N-terminal end, respectively. Spectroscopy and electrochemical techniques, which allowed us to evaluate the thermodynamic parameters of the electrode, surface coverage factor, and chemical stability
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