Abstract
A procedure to measure the serum concentration of glycogen phosphorylase during acute myocardial infarction is presented. This method was based on the synthesis of photoaffinity probes, and used the semiquantitative protein electrophoretic mobility shift technique. Three novel photoaffinity probes bearing different secondary tags were synthesized. Their potency was evaluated in an enzyme inhibition assay against rabbit muscle glycogen phosphorylase a (RMGPa). The inhibitory activity of probe 1 was only 100-fold less potent than the mother compound CP-320626. The photoaffinity labeling experiments were also performed, and a protein with molecular weight (MW) of about 90–100 kDa, which was consistent with the MW of GP, was clearly labeled by probe 1. A semiquantitative evaluation of the GP level in serum with probe 1 was also performed. The results showed that the protein band with a MW of about 90–100 kDa was tagged, and the concentration of the protein in serum was found to be between 25 and 50 ng/mL. Mass spectrometric analysis revealed that alpha-1,4 glucan phosphorylase (GPMM) was well-preserved in the bands.
Highlights
Identification and confirmation of myocardial injury is essential for correct patient care and disposition decision in the emergency department
These are named according to the tissue of their initial description: GPLL, GPMM, and GPBB
GPBB, but notofthe effluxbut of determinations. This is because most studies have the only examined the Continuous efforts Continuous for the development of the methods that can accurately and not theand efflux of GPBB
Summary
Identification and confirmation of myocardial injury is essential for correct patient care and disposition decision in the emergency department. Three GP isoenzymes are present in human tissue These are named according to the tissue of their initial description: GPLL (liver), GPMM (muscle), and GPBB (brain). GP is released from glycogen and enters the bloodstream, which is believed to occur via the T-tubulus system [2] It is considered the most sensitive marker for the diagnosis of acute myocardial infarction (AMI) within 4 h after the onset of chest pain [3]. It is considered to be a specific marker for detection of perioperative myocardial injury in patients undergoing coronary artery bypass grafting [2]
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