Design, synthesis and evaluation in vitro of antioxidant compounds with phenol and thiol moieties

Abstract

It is know that compounds with phenol and thiol moieties have antioxidant activity. Therefore, the aim of this study was to synthetized three compounds containing phenol and thiol groups ((cysteine‐phenol (1), benzothiazole‐phenol (2) and benzothiazole‐phenol (3)) to test then as antioxidant agents. The antioxidant activity evaluation was carried out by DPPH determination and by inactivation of •OH radical generated during the Fenton reaction. The results show that DPPH radical reduction is concetration dependent, the compounds (1) and (2) have higher DPPH radical reducing power (>90%) to 0.102 μM, in comparision with N‐acetylcysteine (NAC) which show 55%. The three compounds inhibit the formation of •OH radical during the Fenton reaction by reducing the signal strength between the adduct formed by •OH and N‐tert‐butyl‐á‐phenylnitrone. In conclussion these compounds have higher antioxidant activity than NAC