Design, synthesis and bioevaluation of dihydropyrazolo[3,4- b]pyridine and benzo[4,5]imidazo[1,2- a]pyrimidine compounds as dual KSP and Aurora-A kinase inhibitors for anti-cancer agents

Abstract

Four series of dihydropyrazolo[3,4- b]pyridines and benzo[4,5]imidazo[1,2- a]pyrimidines were designed and synthesized as dual KSP and Aurora-A kinase inhibitors for anti-cancer agents by introducing some fragments of Aurora-A kinase inhibitors into our KSP inhibitor CPUYL064. A total of 19 target compounds were evaluated by two related enzyme inhibition assays and a cytotoxicity assay in vitro. The results showed that some target compounds could inhibit both enzymes, and several of them showed significant inhibition activity against HCT116 cell line. Despite showing moderate KSP and Aurora-A kinase inhibition, the lead compounds 6a and 6e displayed significant cytotoxic activity in the micromolar range, especially against the HCT116 cell line and HepG2 cell line. The results may be useful for developing a new class of inhibitors having a dual function, KSP inhibition and Aurora-A kinase inhibition, for the treatment of cancer.