Abstract
Traditional self-assembling peptide can form nanofiber scaffolds to meet the challenges of advance biomaterial, cell culture, tissue engineering and regeneration. L-amino acids have been widely used instead of D-amino acids to design nanomaterial since some D-amino acids have toxicity of cells. Here we report that using D-amino acids to design a new D-form self-assembling peptide DSAP-2 and the circular dichroism, atomic force microscopy and scanning electron microscopy show that the peptide can form nanofiber scaffold as well. Furthermore, cell inhibition assay confirmed this D-form peptide show no toxicity of cells that can support cell growth. Fluorescence microscopy results show that cells had less cell apoptosis in the 3D environment and displayed a fast proliferation after cultured for 7days. Peptide’s hydrogel not only formed nano-scaffolds surrounded by cells in a 3-D cell culture, but achieved rapid hemostasis in a rabbit liver wound model. Our study suggests this peptide could be used in the wound and beyond in the future. This work could also inspire us to design more novel D-form self-assembling peptide in biomaterials and biomedical areas.
Highlights
At present, self-assembling peptides have been extensively studied in biomaterials and biomedical areas
We present the molecular model of DSAP-2 (Figure 1A), and we had an interest in what structure this peptide would adopt
The interactions of protein-protein or protein-receptor, the cellular membrane structure, the extracellular matrix (ECM) and ligand play a vital role in cellular activities and Citation: Li M, Xu X, Wang R, Li D, Sun Y, et al (2015) Design Self-Assembling Peptide DSAP-2 for 3d Cell Culture and Rapid Hemostasis
Summary
Self-assembling peptides have been extensively studied in biomaterials and biomedical areas. More and more recent designs have shown their surprising results in structures, properties, functions and even in applications [1] They can form well-ordered nanostructures such as nanofiber, nanotube and nanovesicle. They have multiple advantages, including stability, safety, efficiency, nonbiological, biocompatibility, biodegradability and non-immunogenicity [2]. There have been a few reports that D-form self-assembling peptide did not obtain toxicity to cell stains and was resistant to protease digestion, as a 3D biological matrix for cell culture [6,30,31,32]. We utilized two D-amino acids, D-Ala and D-Asp, which were reported to have toxicity of cells, and designed a new chiral self-assembly peptide DSAP-2. Our study could prompt more designers working in basic medical and clinical studies to find more similar peptides
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