Abstract
A new design of antioxidant, the complex between manganese (Mn) porphyrins and catalase-poly(ethylene glycol) (PEG) conjugates, is reported. Gel filtration chromatography and a Langmuir-type adsorption isotherm proved that the catalase-PEG conjugate formed the complex with the Mn-porphyrin. The resulting complex exhibited significant superoxide dismutase (SOD) and catalase activity. These results suggest that the Mn-porphyrin/catalase-PEG complex with dual enzymatic activity, i.e., SOD and catalase, is promising for a new class of antioxidants.
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