Abstract
AbstractCRISPR-Cas9 is the current choice for genome editing for its versatility and specificity. The Cas9 endonuclease makes double-strand breaks at the target sites, which are repaired via non-homologous end-joining or homologous recombination. So far, various genome editing methods using CRISPR-Cas have been developed for Caenorhabditis elegans. However, repairing a double-strand break via homologous recombination is a crucial step to modify a genome in an error-free manner. Here, we focus on a procedure on how to prepare repair templates for precise genome editing via homologous recombination in C. elegans, which applies to a variety of CRISPR-Cas methods.Key wordsCRISPR-Cas9Repair templateGenome editingHomologous recombination C. elegans
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