Design of Novel Putative Peptide Fragments Derived from Exons of P53 Isoform G and P53 Promoter Region ORF Sequence Targeting HDM2/MDM2-P53 Interaction

Abstract

p53 is a potent endogenous tumour suppressor agent which guards against DNA damage and prevents transformation of normal cells into malignant cells. In most forms of cancers except malignant melanoma, mutation occurs in p53 gene rendering it dysfunctional or suppressed. This research attempts to design novel putative bioactive oligopeptides from tumour suppressor p53 gene sequences using its promoter sequence and deriving several primer-based oligomers from the exons of p53 gene. p53 induces cells to undergo repair or apoptosis in response to DNA-damage induced stress and thus acts as the Warden of the Cell. We devise some novel methods to derive our de novo sequences from the exons of this gene and report discovery of two new oligopep-tides such as HRGRES and PAAPA (P 7 TA 7) peptide being two putative oligopeptides.