Abstract
Methods C56BL/6 mice were sensitized by intranasal administration of olive extract+cholera toxin B, for 6 weeks. Then, mice received immunotherapy (IT) treatment by subcutaneous (sc) injection of dendrimer-Ole1±50 mg of CpG, for 8 weeks. Seven days after the IT, mice were challenged with 100 mg of olive extract (ip). Severity of anaphylaxis was measured by drop in body temperature and the humoral response by ELISA.
Highlights
Polymers containing immunogenic peptides bonded to dendrimeric structures have been developed to be used for vaccines in cancer, infectious diseases, and allergy
Severity of anaphylaxis was measured by drop in body temperature and the humoral response by ELISA
Olive sensitized mice treated with the dendrimer-Ole1 without CpG developed a drop in body temperature similar to anaphylactic mice (35.02±1.39 vs 34.48±0.82, respectively), indicating that Ole e 1 within a dendrimeric structure is recognized in vivo
Summary
Polymers containing immunogenic peptides bonded to dendrimeric structures have been developed to be used for vaccines in cancer, infectious diseases, and allergy. Our aim is to design a dendrimeric structure containing Ole e 1 and CpG, in order to modulate an allergic immune response towards Th1, in an experimental model of anaphylaxis
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