Abstract
Glycine max (L.) Merrill is a crop that brings a lot of economic and nutritive values, however soybean is quite sensitive to stress. The applications of gene technology can improve resistance of soybean plants against external stress factors. The aim of this study was to conduct a transgenic vector containing GmDREB6 gene and determine the expression of gene encoding GmDREB6 in Nicotiana tabacum before transforming into soybean plants. The GmDREB6 artificial gene was synthesized containing nucleotide fragment encoding 230 amino acids, nucleotide fragment encoding cmyc antigen and nucleotide fragments with cut-off points of XbaI/SacI enzyme pair. The results indicated that the 35S-GmDREB6-cmyc construct was designed and transferred into tobacco plants. The GmDREB6 was incorporated in the genome and was expressed in transgenic tobacco plants at the transcriptional level. The transgenic vector pBI121_GmDREB6 well worked on the model tobacco plants. Therefore, it can be used for transferring into soybean plants to enhance soybean tolerance to abiotic stress.
Highlights
Soybean (Glycine max (L.) Merrill) brings economic, nutritive value and can improve soil fertility
We present design results of the construct carrying GmDREB6 genes and initially evaluate the activities of this structure on the model of tobacco plants
Based on information of GmDREB6 isolated from DT2008 Vietnamese soybean cultivar and GmDREB6 with EF551166 code on GenBank (Liu et al, 2007) [6], artificial GmDREB6 gene was designed with 741 bp in size and nucleotide sequence of GmDREB6 gene as follows: 5’gctctagaATGGTCATGGAAGAATCTAACCCATTAGACAATG AAGTTCAACCAACCACTTCATCATCCTCTTTCACCATTACTACTCC CTCTACCTCCTCTTCTTCCTCCATAGAAGAAGCCACTAACACTAC AAAGGAGAAAAAGAAGAAAGCCAATAGTAATGAAGGCAAGCACCC TACGTATAGAGGAGTTCGCATGCGTCAATGGGGCAAATGGGTATC CGAAATTAGAGAGCCAAGGAAGAAATCAAGAATTTGGCTTGGTAC TTTTCCCACGCCCGATATGGCGGCTCGAGCCCATGACGTGGCGGC TCTCACGATCAAAGGCAGCTCGGCTTACCTCAATTTCCCAGAATT AGCCGACGAGCTGCCCCGCCCCGCCAGCACCTCCCCCAAGGACAT CCAAGCTGCGGCGTCTAAAGCCGCTGCCTTGGATTTCGGCCACCG AAGCCATGACGCCGAGTCCGAGCTGAGCCGAGCCGTTTCCTCCTC AATCCTCACACAAGCATCATCATCATCATCCAATTCATCATCGTT GAAGGGTGTGGATGACACATTCTTGGACCTTCCTGATCTCTCTCT CGACTTGAGCCATGGAGCCGATGAGTTTCATTATTCGTCAGCTTG GCTTGTAGCCGGAGCCGAACATATAGAGCTGGGTTTTCGGCTTGA GGATCCTTTTCTATGGGAATCATATGAACAAAAACTCATCTCAGA AGAGGATCTGAATTAAgagctcg 3’
Summary
Soybean (Glycine max (L.) Merrill) brings economic, nutritive value and can improve soil fertility. Some studies on improvement biotic and abiotic stress tolerance of crops and soybeans using gene transfer have been reported [1]-[5]. The 35SGmDREB2-cmyc construct carrying GmDREB2 gene was designed and transformed into Nicotiana tabacum [2] According to these authors, the proline accumulation of the transgenic tobacco lines was higher than on non-transgenic plants during drought conditions. DREB (dehydrationresponsive element binding) transcription factor has been identified and its function is to activate genes involved in abiotic stress response. Trans-factors bind to cis-elements to activate expression of functional genes in plants under stressed conditions. We present design results of the construct carrying GmDREB6 genes and initially evaluate the activities of this structure on the model of tobacco plants.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
