Design of antibiofilm surfaces by immobilization of biogenic silver nanoparticles on amine self-assembled monolayers

Abstract

Hospital-acquired infections (HAIs) are a serious medical threat nowadays, compromising the efficacy of the existing medical infrastructure. HAIs driven impact on the antibiotics usage resulted in further complications of (a) developing multi-drug resistant bacterial strain and (b) insignificant potential towards eradicating biofilm formation. To address this serious issue, we have developed silver nanoparticles (AgNPs) based antimicrobial surfaces using amine self-assembled monolayers. The biogenic spherical AgNPs (76 ± 15 nm) were synthesized using a plant extract of Miscanthus khasiana (silver grass) as a reducing agent. The surface energy of AgNPs-amine surface was 38.2 mJ/m2 and remained constant after the in-vitro stability test. The prepared surfaces hampered the bacterial adhesion and biofilm formation of Pseudomonas aeruginosa. A 67% reduction in the viability of the adhered bacteria was observed in the presence of the designed AgNPs-amine surface, confirming its antibiofilm potential.