Abstract
We have implemented a hybrid Expert System, that combines experts rules and mathematical correlations to manipulate physicochemical databases for selecting the optimum sequence of operations for purification of proteins. Two criteria were implemented to select the optimum sequence of purification. One of these uses the selection separation coefficient (SSC) and the other uses the final level of purity. The sequences suggested by the expert system have been investigated experimentally using both criteria and it has been shown that both are valid for practical application, but the sequences suggested by the Purity criteria have fewer steps than those suggested by the SSC criteria.
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